Homogenyze the plant tissue
Prepare plant material for enzymatic digestion.
ProtoSep
Automated Protoplast Isolator ProtoSep
Next-generation automated device for rapid, gentle, and reproducible protoplast isolation.
A new automated, patent-pending solution for protoplast isolation
ProtoSep is built to let the device handle the complex isolation work while researchers focus on their science. Its automated workflow supports tissue digestion, purification, washing, and final recovery in one streamlined process.
- Increased Efficiency: automates the entire process from tissue digestion to final purification.
- Optimized Lab Schedules: reduces manual intervention and frees up researcher time.
- Cost Reduction: minimizes waste and maximizes resource utilization.
- Simplified Protocol: sets a new benchmark for high-throughput plant biotechnology workflows.
Device use
Streamlined from sample loading to ready-to-use cells
The Automated Protoplast Isolator ProtoSep operates through a workflow designed for maximum efficiency and reproducibility. The integrated microfluidics system helps deliver consistent, high-quality results.
Mix with enzymes
Combine the prepared tissue with the enzyme solution.
Load the sample
Place the sample into the ProtoSep workflow.
Run the protocol
Let ProtoSep control the isolation process.
Applications
Built for modern plant biotechnology workflows
Consistent protoplast isolation
Standardize digestion, washing, and recovery conditions for more reproducible isolation outcomes.
Single-cell analysis preparation
Prepare isolated protoplasts for advanced single-cell studies.
Plant transformation studies
Enable efficient genetic transformation experiments.
Regeneration experiments
Support plant regeneration and tissue culture research.
Transcriptomics preparation
Prepare protoplast material for single-cell, bulk, and spatial transcriptomics workflows.
Advanced plant cell research
Support controlled experiments with isolated plant cells for research and development.
Comparison
Comparison of protoplast isolation methods
| ProtoSep | Manual | |
|---|---|---|
| Skill needed | Low | High |
| Hands-on time | 30 min | 6 h |
| Cost of personnel | 15 EUR | 180 EUR |
| Automation capability | High | Low |
| Experiment scheduling | High | Low |
| Reproducibility | High | Low |
Use case: lettuce
From seedlings to regenerating cultures
In lettuce, ProtoSep has been used as part of a workflow from seed germination and tissue preparation through protoplast isolation and regeneration culture.
14 daysSeed germination before tissue preparation
1 g tissue14-day-old lettuce shoots prepared for isolation
~7MProtoplasts isolated from 1 g of lettuce shoots
ShootsRegeneration progression tracked after isolationEvaluate ProtoSep
Run a trial with your plant system
Choose a demo in your lab or send starting material to ours. In both cases, the goal is practical: a trial run with raw data and statistics you can inspect.