Enzyme mix
Pre-weighed cellulase and macerozyme blend in optimized ratios for efficient cell wall digestion.
Protoplast isolation kit
A faster start to reproducible protoplast isolation.
Ready-to-use reagents for plant protoplast isolation, with pre-weighed enzymes, prepared buffers and a clear bench protocol.
What's inside
Three prepared components, one cleaner workflow
The kit includes the core reagents and a visual bench protocol for a consistent digestion and purification workflow. One kit supports up to 50 standard isolations.
Enzyme buffer
Osmotically balanced digestion buffer with pre-adjusted pH for high protoplast viability.
Gradient buffer
Purification buffer for cleaner separation of intact protoplasts from debris and undigested tissue.
~20 min preparation time
Dissolve enzymes
Reconstitute the pre-weighed enzyme mix.
Filter
Prepare a clean enzyme solution using standard filtration.
Freeze
Aliquot and store for predictable use in future experiments.
Proof and data
Higher yield with fewer preparation variables
In lettuce leaf workflows, the pre-optimized kit improved recovered protoplast yield compared with freshly prepared DIY enzyme solutions while keeping viability in the range required for downstream transformation and analysis.
Average recovered protoplast yield per 1 g of lettuce leaf tissue across representative mesophyll isolations.
Kit vs DIY
Pre-optimized preparation versus manual setup
| Our pre-optimized kit | Traditional DIY method | |
|---|---|---|
| Preparation time | Approx. 20 minutes | Approx. 1 hour for enzyme solution and buffers |
| Reagent preparation | Pre-weighed enzymes and pre-adjusted buffers | Manual weighing, calculations and pH adjustment |
| Consistency | Controlled reagent variables | Batch-to-batch variance from manual preparation |
| Required equipment | Standard syringe filter and basic labware | Analytical balance, calibrated pipettes and pH meter |
FAQ
Common questions
Compatibility and performance
Which plant species can the kit be used with?
The kit is designed for plant protoplast isolation workflows across species. Tissue preparation, digestion time and other protocol parameters may need optimization for each species and tissue type. The performance data shown on this page come from lettuce leaf tissue.
Which downstream techniques are supported?
Protoplasts isolated with the kit are intended for transient transformation, regeneration workflows, microscopy, viability assays, bulk transcriptomics and single-cell preparation.
What is the typical digestion time?
Digestion time depends on the species, tissue condition and experimental setup. For lettuce leaf mesophyll, the workflow typically runs between 3 and 6 hours.
Technical specifications and storage
How many isolations does one kit support?
One kit supports up to 50 standard isolations.
Are filters, tubes and dishes included?
No. The kit is designed to work with standard sterile syringe filters, tubes, dishes and pipette tips already used in plant cell biology labs.
How is the kit stored and shipped?
The entire kit is shipped at room temperature. The kit has a 6-month shelf life when stored as recommended, and reconstituted enzyme solution can be aliquoted and frozen for repeated use.
Sample kit
Ready to test the kit in your workflow?
Request a sample kit for your plant workflow, or tell us which species and tissue type you work with.
Request a sample kit